C2an36787d 1619..1627
نویسندگان
چکیده
Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.
منابع مشابه
Implementation and analysis of Public Law 98-460--section 1619 (the Social Security Disability Benefits Reform Act of 1984).
The following is a reprint of the report to Congress on section 1619 of the Social Security Act prepared by the Department of Health and Human Services in response to a provision of the Social Security Disability Benefits Reform Act of 1984 (Public Law 98-460). It also includes some of the report's appendix material. In addition to calling for an evaluation of the provision's effectiveness, tha...
متن کاملPrecise Localization of the Soft Gamma Repeater SGR 1627−41 and the Anomalous X-ray Pulsar AXP 1E1841−045 with Chandra
We present precise localizations of AXP 1E1841−045 and SGR 1627−41 with Chandra. We obtained new infrared observations of SGR 1627−41 and reanalyzed archival observations of AXP 1E1841−045 in order to refine their positions and search for infrared counterparts. A faint source is detected inside the error circle of AXP 1E1841−045. In the case of SGR 1627−41, several sources are located within th...
متن کاملSuppression of human detrusor smooth muscle excitability and contractility via pharmacological activation of large conductance Ca2+-activated K+ channels.
Overactive bladder syndrome is frequently associated with increased detrusor smooth muscle (DSM) contractility. We tested the hypothesis that pharmacological activation of the large-conductance voltage- and Ca(2+)-activated K(+) (BK) channel with NS-1619, a selective BK channel opener, reduces the excitability and contractility of human DSM. We used the amphotericin-perforated whole cell patch-...
متن کاملRemission of bullous pemphigoid after rituximab treatment in a psoriasis patient on regular low-dose methotrexate.
متن کامل
Reverse electron flow-induced ROS production is attenuated by activation of mitochondrial Ca2+-sensitive K+ channels.
Mitochondria generate reactive oxygen species (ROS) dependent on substrate conditions, O(2) concentration, redox state, and activity of the mitochondrial complexes. It is well known that the FADH(2)-linked substrate succinate induces reverse electron flow to complex I of the electron transport chain and that this process generates superoxide (O(2)(*-)); these effects are blocked by the complex ...
متن کامل